Aminoglycoside hydrogel microbeads and macroporous gels with chemical crosslink, method of preparation and use thereof
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsKaushal Rege, Taraka Sai Pavan Grandhi, Thrimoorthy Potta
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Abstract
Methods and materials for the generation of amikacin antibiotic-derived microbeads, (FIG. 3). These beads may function as anion-exchange resins for use in pDNA purification as well as in situ capture of DNA from mammalian cells. New microresin and macroporous monolith based materials also are disclosed and may function for plasmid DNA binding and purification, mammalian whole cell genomic DNA extraction, and in-vitro cell culture.
Claims22
A method to prepare a macroporous column, comprising:
- providing a chromatographic column packed with salt;
- mixing an aminoglycoside and a cross-linker in an organic solvent;
- disposing said aminoglycoside/cross-linker/organic solvent in said chromatographic column;
- after said aminoglycoside reacts with said cross-linker to form a macroporous porous, cross-linked resin, adding water to said column to dissolve said salt;
- draining said water from said chromatographic column,
A macroporous, cross-linked resin, formed by the process of:
- providing a chromatographic column packed with salt;
- mixing an aminoglycoside and a cross-linker in an organic solvent;
- disposing said aminoglycoside/cross-linker/organic solvent in said chromatographic column;
- after said aminoglycoside reacts with said cross-linker to form a macroporous porous, cross-linked resin, adding water to said column to dissolve said salt;
- draining said water from said chromatographic column.
A method to form microbeads comprising an aminoglycoside moiety, comprising:
- polymerizing a reaction mixture comprising an aminoglycoside and a cross-linker;
- prior to gellation, disposing said reaction mixture into heated mineral oil;
- collecting said microbeads by centrifugation.
Microbeads comprising an aminoglycoside moiety, formed by the process of:
- polymerizing a reaction mixture comprising an aminoglycoside and a cross-linker;
- prior to gellation, disposing said reaction mixture into heated mineral oil;
- collecting said microbeads by centrifugation.
Dependencies are inferred from the claim text.