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US 2018/0021696 A1Published application
Affinity chromatography wash buffer
AssigneeCehpalon, Inc.
InventorsLu WANG, Tim ZHOU, Zhaoqing ZHANG, Mi JIN
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AssigneeCehpalon, Inc.
InventorsLu WANG, Tim ZHOU, Zhaoqing ZHANG, Mi JIN
Estimating the term…
A wash buffer comprising greater than 0 mM and less than about 500 mM arginine, greater than 0 mM and less than about 250 mM guanidine, greater than 0 mM and less than about 250 mM sodium chloride, and greater than 0 mM and less than about 50 mM of an anionic surfactant, or greater than 0% and less than about 0.25% w/v of a non-ionic surfactant. When used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly reduces the level of host cell proteins from the preparation. Following affinity chromatography with the wash buffer, the protein of interest may be further purified using membrane chromatography.
An affinity chromatography wash solution, comprising water, a basic amino acid, and guanidine.
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A composition, comprising an aqueous carrier, a recombinantly-expressed or hybridoma-expressed antibody that specifically binds to:
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A composition, comprising an aqueous carrier, a recombinantly-expressed or hybridoma-expressed antibody that specifically binds to calcitonin gene-related peptide (CGRP), and a level of host cell proteins at a level of from about 400 ppm to about 800 ppm of the composition.
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A composition, comprising an aqueous carrier, a recombinantly-expressed or hybridoma-expressed antibody that specifically binds to CD38, and a level of host cell proteins at a level of from about 50 ppm to about 500 ppm of the composition.
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A method of preparing a purified protein of interest, comprising loading a mixture comprising a protein of interest and one or more contaminant proteins onto an affinity chromatography ligand, washing the ligand with an aqueous wash solution comprising a basic amino acid and guanidine to elute the one or more contaminant proteins from the ligand, and then eluting the protein of interest from the ligand, thereby forming a purified eluate of the protein of interest.
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Dependencies are inferred from the claim text.