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US 2018/0020658 A1Published application
Enhancement of cell cryopreservation with glycolipids
AssigneeTissue Testing Technologies LLC
InventorsLia H. CAMPBELL, Kelvin GM BROCKBANK
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Abstract
Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one glycolipid, and then subjecting the cellular material to a preservation protocol.
Claims20
A method for preserving living cellular material, comprising:
- exposure of the cellular material to medium containing at least one glycolipid and a cryoprotectant for a predetermined amount of time;
- and
- after the exposure, subjecting the cellular material to a preservation protocol comprising cryopreservation at a cryopreservation temperature of about −80° C. or less.
Cryopreserved cells obtained by exposure of a living cellular material to medium containing at least one glycolipid and a cryoprotectant for a predetermined amount of time;
- and
- after the exposure, subjecting the cellular material to a preservation protocol;
- wherein a cell viability (%) of the cellular material after the preservation protocol is at least 60%.
A method for increasing production yield of viable cryopreserved cellular material, comprising:
- exposure of a cellular material to medium containing at least one glycolipid and a cryoprotectant for a predetermined amount of time to form a cryopreservation composition;
- subjecting the cryopreservation composition to a preservation protocol comprising cryopreservation at a cryopreservation temperature of about −80° C. or less, wherein
- the preservation protocol comprises cooling the cellular material at a cooling rate greater than about −6.0° C. per minute;
- and
- after completion of the preservation protocol, recovering the cryopreserved cellular material;
- wherein a cell viability (%) of the recovered cryopreserved cellular material is at least 60%.
3 more elements
Dependencies are inferred from the claim text.