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US 2018/0020646 A1Published application
Gene knockout method
AssigneeRiken
InventorsHiroki UEDA, Genshiro SUNAGAWA, Kenta SUMIYAMA, Maki UKAI, Dimitri PERRIN
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Abstract
A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.
Claims13
A method for producing a cell in which a target gene is knocked out,
- the method comprising the step of:
- introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein.
Dependencies are inferred from the claim text.