Rice plants with altered seed phenotype and quality
AssigneeInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
InventorsXiangdong FU, Shaokui WANG, Kun WU, Qian LIU, Shan LI
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Abstract
The invention relates to plants with an improved organ phenotype. In particular, the invention relates to rice plants which have better grain quality and altered seed phenotype due to increased expression of GW7 (LOC_Os07g41200) or due to a combination of increased expression of GW7 and reduced expression of GS3 (OsSPL16). Grains show increased length and increased slenderness.
Claims45
A plant wherein the expression of a nucleic acid sequence encoding a GW7 polypeptide or the activity of a GW7 polypeptide is increased.
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A vector comprising an isolated nucleic acid comprising a OsGW7 nucleic acid sequence comprising or consisting of SEQ ID NO:
- 1 or 2.
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A method for altering the phenotype of a plant organ said method comprising increasing the expression of a nucleic acid sequence encoding a GW7 polypeptide or increasing the activity of a GW7 polypeptide.
A method for producing a genetically engineered plant said method comprising increasing the expression of a nucleic acid sequence comprising or consisting of SEQ ID NO:
- 1 or 2 or a functional rice variant thereof or increasing the activity of a GW7 polypeptide comprising or consisting of SEQ ID. NO:
- 3, a homolog or a functional rice variant thereof or comprising introducing a mutation in the promoter or coding region of a GW7 nucleic acid sequence comprising or consisting of SEQ ID. No 1 or 2, a homolog or functional variant thereof.
A method for identifying and/or selecting a rice plant of a rice germplasm that has a phenotype selected from increased length of grain or other organs and increased slenderness grain or other organs, comprising detecting in the first rice plant or the rice germplasm at least one polymorphism within a marker locus that is associated with said phenotype of said rice plant or said rice germplasm, wherein said marker locus is genetically linked with a chromosomal region comprising nucleic acid sequence SEQ ID NO:1, and wherein the rice plant or a progeny thereof or the first rice germplasm or a progeny thereof is selected.
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A method of identifying alleles in rice plants or rice germplasm that are associated with improved grain quality and/or increased yield, the method comprising:
- a. obtaining a population of rice plants, wherein one or more plants exhibit improved grain quality;
- b. evaluating allelic variations with respect to the polynucleotide sequence encoding a protein comprising a polypeptide comprising:
- SEQ ID NO:
- 3 or in the genomic region that regulates the expression of the polynucleotide encoding the protein;
- c. obtaining phenotypic values of improved grain quality for a plurality of rice plants in the population;
- d. associating the allelic variations in the genomic region associated with the polynucleotide with the phenotype;
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- e. identifying the alleles that are associated with improved grain quality.
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Dependencies are inferred from the claim text.