Methods, reagents and cells for biosynthesizing compounds
AssigneeInvista North America S.A R.L.
InventorsAlex Van Eck Conradie, Adriana Leonora Botes, Ramdane Haddouche
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Abstract
This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol.
Claims26
1-83. (canceled)
A method of producing 7-hydroxyheptanoate methyl ester in a recombinant host via fermentation, said method comprising:
- enzymatically converting heptanoate to heptanoate methyl ester using a polypeptide having fatty acid O-methyltransferase activity;
- and
- enzymatically converting heptanoate methyl ester to 7-hydroxyheptanoate methyl ester using a polypeptide having monooxygenase activity.
A recombinant host comprising at least one exogenous nucleic acid encoding a polypeptide having monooxygenase activity and at least one nucleic acid encoding a polypeptide having fatty acid O-methyltransferase activity, wherein:
- said polypeptide having monooxygenase activity has at least 70% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 28, or SEQ ID NO:
- 29;
- and
- said polypeptide having fatty acid O-methyltransferase activity has at least 70% sequence identity to an amino acid sequence set forth in SEQ ID NO:
- 23, SEQ ID NO:
- 24, or SEQ ID NO:
- 25,
- and said recombinant host produces 7-hydroxyheptanoate methyl ester.
Dependencies are inferred from the claim text.