Method for detecting analyte

A method for distinctly detecting in a sample a first analyte and a second analyte of a different kind of the first analyte, the method comprising the steps of:

• forming on each of carrier particles a first complex containing a first capture substance capable of binding to a first analyte, one molecule of the first analyte, a second capture substance capable of binding to the first analyte, and a catalyst;
• forming on each of carrier particles a second complex containing a third capture substance capable of binding to a second analyte, one molecule of the second analyte, a fourth capture substance capable of binding to the second analyte, and a catalyst;
• immobilizing a reaction product on each of the carrier particles by reacting the catalyst in the compolex with a substrate;
• and
• distinctly detecting the first analyte and the second analyte by detecting the carrier particles on each of which the reaction product is immobilized,
• wherein
• the formation step comprises a step of mixing the sample and a reagent containing a plurality of the carrier particles;
• one molecule of either of the first analyte and the second analyte is captured per one carrier particle by the formation step;
• the reaction product is immobilized on each of the carrier particles on each of which the catalyst that has produced the reaction product is immobilized, but is not substantially immobilized on another carrier particle in the immobilization step;
• carrier particles on each of which the first analyte is immobilized and carrier particles on each of which the second analyte is immobilized generate mutually distinguishable signals by the catalytic reaction;
• each carrier particle is not compartmentalized in the detection step;
• each carrier particle is not compartmentalized in the immobilization step;
• and
• the first analyte and the second analyte are distinctly detected in the detection step based on the mutually distinguishable signals.

A method of digital detection of an analyte in a sample, the method comprising the steps of:

• forming on each of carrier particles a complex containing a first capture substance capable of binding to an analyte, one molecule of the analyte, a second capture substance capable of binding to the analyte, and a catalyst;
• immobilizing a reaction product on each of the carrier particles by reacting the catalyst in the compolex with a substrate;
• and
• detecting the analyte by digitally detecting the carrier particles on each of which the reaction product is immobilized,
• wherein
• one molecule of the analyte is captured per one carrier particle in the formation step;
• the reaction product is immobilized on the carrier particles on each of which the catalyst that has produced the reaction product is immobilized, but is not substantially immobilized on another carrier particle in the immobilization step;
• the formation step comprises a step of mixing the sample and a reagent containing a plurality of the carrier particles;
• and
• each carrier particle is not compartmentalized in the detection step.