Assay plates, reader systems and methods for luminescence test measurements

A multi-well plate comprising:

• (a) a first electrically conductive layer partitioned into two or more electrically isolated sectors;
• (b) an insulating layer having a plurality of insulating layer openings;
• (c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings;
• and
• (d) a plate top having a plurality of plate top openings;
• wherein said insulating layer is between said first electrically conductive layer and said plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells and wherein said two or more electrically isolated sectors are aligned with said plate top openings thereby forming independently addressable sectors of jointly addressable wells.

A multi-well plate for conducting electrode induced luminescence assays comprising:

• (a) a first electrically conductive layer;
• (b) an insulating layer having a plurality of insulating layer openings;
• (c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings, said conductive film being partitioned into two or more electrically isolated sectors;
• and
• (d) a plate top having a plurality of plate top openings;
• wherein said insulating layer is between said first electrically conductive layer and plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells for conducting said electrode induced luminescence assays.

A multi-well plate for conducting electrode induced luminescence assays comprising:

• (a) a first electrically conductive layer partitioned into two or more electrically isolated sectors;
• (b) an insulating layer having a plurality of insulating layer openings;
• (c) a second electrically conductive layer on said insulating layer having a plurality of second electrically conductive layer openings, said conductive film being partitioned into two or more electrically isolated sectors;
• and
• (d) a plate top having a plurality of plate top openings;
• wherein said insulating layer is between said first electrically conductive layer and plate top and wherein said insulating layer openings, said second electrically conductive layer openings and said plate top openings are aligned forming wells for conducting said electrode induced luminescence assays.

A multi-well plate bottom comprising:

• (a) a substrate having a top surface and a bottom surface;
• (b) a plurality of patterned working electrodes on said top surface;
• (c) a plurality of patterned counter electrodes on said top surface each of said patterned counter electrodes being associated with corresponding patterned working electrodes;
• and
• (d) two or more independently addressable sectors, each sector having two or more independently addressable contacts on said bottom surface, each of said contacts corresponding to one or more electrodes within wells within one of said sectors.

A multi-well plate bottom comprising:

• (a) a substrate having a top surface and a bottom surface;
• (b) a plurality of patterned working electrodes on said top surface;
• (c) a plurality of patterned counter electrodes on said top surface, each of said patterned counter electrodes being associated with corresponding patterned working electrodes;
• and
• (d) two or more independently addressable contact sectors on said bottom surface, each of said contact sectors corresponding to an electrode sector comprising one or more of said plurality of patterned working electrodes and one or more of said plurality of patterned counter electrodes on said top surface and including a plurality of conductive contact surfaces,
• wherein said plurality of conductive contact surfaces include:
• (i) a first conductive contact surface located within a first contact region, said first conductive contact surface being electrically connected to said one or more corresponding patterned working electrodes on said top surface;
• and
• (ii) a second conductive contact surface located within a second contact region, said second conductive contact surface being electronically connected to said one or more corresponding patterned counter electrodes on said top surface;
• wherein said first conductive contact surface and said second conductive contact surface are electrically isolated from each other.