Methods of membrane-based proteomic sample preparation
AssigneeChildren's Medical Center Corporation
InventorsHanno STEEN, Sebastian T. BERGER, Saima AHMED
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Abstract
A method for rapid isolation of a biological compound (e.g. protein) from an aqueous sample is described herein. The method uses a porous hydrophobic membrane that has an average pore size significantly greater than the size of the biological compound. The method permits the biological compound to attach to the membrane while the aqueous solvent rapidly moves through the membrane under the application of a vacuum. The biological compound that is attached to the membrane can be washed, optionally digested, and eluted for analysis.
Claims27
A method of separating a biological compound from an aqueous sample containing the biological compound, the method comprising:
- (i) introducing the aqueous sample to a well of a plate, wherein the well has a bottom comprising a porous hydrophobic membrane, and the sample is in contact with a first side of the porous hydrophobic membrane;
- (ii) applying a vacuum to a second side of the porous hydrophobic membrane, thereby drawing the aqueous sample through the porous hydrophobic membrane, wherein the biological compound associates with the porous hydrophobic membrane as aqueous solvent passes through;
- and
- (iii) introducing a solvent solution to the first side of the porous hydrophobic membrane to elute the biological compound from the porous hydrophobic membrane.
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Dependencies are inferred from the claim text.