Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Anti-Mycoplasma hyorhinis IgG in Swine Serum
AssigneeMerial Limited
InventorsPaulraj Lawrence, Russell F. Bey, Mike Stoll, Eric Bumgardner, Douglas Stine
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Abstract
This disclosure presents an Enzyme-Linked Immunosorbent Assay (ELISA) for the selective detection of anti-Mycoplasma hyorhinis IgG in porcine serum, which may contain antibodies specific to multiple other Mycoplasma spp.
Claims10
A purified, recombinant, non-naturally occurring, non-post-translationally modified, Mycoplasma hyorhinis (M. hyorhinis) p37 polypeptide having a sequence with at least 98% identity to the sequence as set forth in SEQ ID NO:5;
- wherein the polypeptide is useful for the selective detection of M. hyorhinis antibodies.
A diagnostic kit for the selective diagnosis of the presence in a biological sample of M. hyorhinis P37 antibodies;
- wherein even if the biological sample contains M. hyosynoviae and M. hyopneumoniae antibodies against orthologous, homologous or paralogous P37, the kit will not yield a positive result unless M. hyorhinis antibodies are present.
A method of detecting exposure to M. hyorhinis in an animal or in a biological sample from said animal, the method comprising the step of detecting the presence or absence of an immune response to the polypeptide having the amino acid sequence shown as SEQ ID NO:5.
Dependencies are inferred from the claim text.