Compositions, kits, and methods to detect hiv virus

A composition, comprising:

• a primer having the nucleic acid sequence of ACeIN-F3_c;
• a primer having the nucleic acid sequence of ACeIN-B3a;
• a primer having the nucleic acid sequence of ACeIN-B3b;
• a primer having the nucleic acid sequence of ACeIN-FIPe;
• a primer having the nucleic acid sequence of ACeIN-FIPf;
• a primer having the nucleic acid sequence of ACeIN-BIP-song;
• a primer having the nucleic acid sequence of ACeIN-LF;
• and
• a primer having the nucleic acid sequence of ACeIN-LB.

A method of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising:

• contacting a reaction mixture comprising a reverse transcription-based loop mediated isothermal amplification assay composition comprising a set of primers comprising a primer having the nucleic acid sequence of ACeIN-F3_c;
• a primer having the nucleic acid sequence of ACeIN-B3_a;
• a primer having the nucleic acid sequence of ACeIN-B3_b;
• a primer having the nucleic acid sequence of ACeIN-FIP_e;
• a primer having the nucleic acid sequence of ACeIN-FIP_f:
• a primer having the nucleic acid sequence of ACeIN-BIP;
• a primer having the nucleic acid sequence of ACeIN-LF;
• and a primer having the nucleic acid sequence of ACeIN-LB or a set of primers comprising a primer having the nucleic acid sequence of ACeIN-F3_c;
• a primer having the nucleic acid sequence of ACeIN-B3a;
• a primer having the nucleic acid sequence of ACeIN-B3b;
• a primer having the nucleic acid sequence of ACeIN-FIPe;
• a primer having the nucleic acid sequence of ACeIN-FIPf;
• a primer having the nucleic acid sequence of ACeIN-BIP-song;
• a primer having the nucleic acid sequence of ACeIN-LF;
• and a primer having the nucleic acid sequence of ACeIN-LB, magnesium, dNTPs, a reaction buffer, and a DNA polymerase and a sample to be tested for presence of HIV nucleic acids;
• incubating the reaction mixture and the sample under DNA polymerase reactions conditions so as to produce a reaction product comprising amplified HIV nucleic acids;
• and
• detecting the reaction product.

A method of monitoring a response to a medication in a subject in need thereof, comprising:

• obtaining a first sample from the subject at a first time point;
• obtaining a second sample from the subject a second time point following administration of a medication to the subject;
• determining the amount of human immunodeficiency virus (HIV) in the first and second samples, the determining comprising
• performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample containing HIV using a set of primers comprising a primer having the nucleic acid sequence of ACeIN-F3_c;
• a primer having the nucleic acid sequence of ACeIN-B3_a;
• a primer having the nucleic acid sequence of ACeIN-B3_b:
• a primer having the nucleic acid sequence of ACeIN-FIP_e;
• a primer having the nucleic acid sequence of ACeIN-FIP_f;
• a primer having the nucleic acid sequence of ACeIN-BIP;
• a primer having the nucleic acid sequence of ACeIN-LF;
• and a primer having the nucleic acid sequence of ACeIN-LB or a set of primers comprising a primer having the nucleic acid sequence of ACeIN-F3_c:
• a primer having the nucleic acid sequence of ACeIN-B3a;
• a primer having the nucleic acid sequence of ACeIN-B3b;
• a primer having the nucleic acid sequence of ACeIN-FIPe;
• a primer having the nucleic acid sequence of ACeIN-FIPf;
• a primer having the nucleic acid sequence of ACeIN-BIP-song;
• a primer having the nucleic acid sequence of ACeIN-LF;
• and a primer having the nucleic acid sequence of ACeIN-LB;
• and
• comparing the amount of HIV in the first and second samples,
• wherein a decrease in the amount of HIV from the first sample relative to the second sample indicates treatment of HIV infection.