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AssigneesCodexis, Inc., The Coca-Cola Company
InventorsJonathan Vroom, Oscar Alvizo, Pankaj Soni, Michael D. Brown, David Entwistle
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The present invention provides an enzymatic means for the biosynthetic production of caffeine. The present invention provides biosynthetic methods for production of caffeine comprising: providing guanine, a guanine deaminase, at least one methyl transferase, and a methyl donor; contacting the guanine with the gtheuanine deaminase to produce xanthine; contacting the xanthine with the methyl transferase and a methyl donor, under conditions wherein the xanthine is methylated, to produce a monomethylxanthine; contacting the monomethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the monomethylxanthine is methylated, to produce a dimethylxanthine; and contacting the dimethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the dimethylxanthine is methylated, to produce caffeine (i.e., 1,3,7-trimethylxanthine).
A biosynthetic method for production of caffeine comprising:
A biosynthetic method for production of caffeine comprising:
A biosynthetic method for the production of caffeine comprising:
A non-naturally occurring polynucleotide sequence encoding a guanine deaminase, wherein said polynucleotide is codon-optimized and selected from SEQ ID NOS:2, 4, 6, 8, 10, 12, 14, 16, and 18.
A non-naturally occurring polynucleotide sequence encoding a methyl transferase, wherein said polynucleotide is codon-optimized and selected from SEQ ID NOS:24, 26, 28, 30, 32, 34, 46, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, and 82.
Dependencies are inferred from the claim text.