Methods for producing abienol
AssigneeDsm IP Assets B.V.
InventorJohn ROYER
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Abstract
The present disclosure relates to a novel method, expression vectors, and host cells for producing abienol by converting geranylgeranyl diphosphate (GGPP) to abienol in the presence of a combination of a class II diterpene synthase and a bifunctional class I/II abienol synthase.
Claims22
A method for producing abienol, which comprises converting geranylgeranyl diphosphate (GGPP) to abienol in the presence of a combination of class II diterpene synthase (diTPS) and bifunctional class I/II abienol synthase (CAS).
A recombinant host cell comprising a class II diterpene synthase and bifunctional class I/II abienol synthase.
An expression vector comprising a polynucleotide molecule encoding an amino acid sequence comprising SEQ ID NO:1 and a polynucleotide molecule encoding an amino acid sequence comprising SEQ ID NO:3.
An expression vector comprising a polynucleotide molecule encoding an amino acid sequence comprising SEQ ID NO:2 and a polynucleotide molecule encoding an amino acid sequence comprising SEQ ID NO:3.
Dependencies are inferred from the claim text.