Cell culture method and cell culture apparatus
AssigneeToyo Seikan Group Holdings, Ltd.
InventorsNorihiko HATA, Masahiro MURAI
Life of this patent
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Abstract
A bottom surface of a container (1) formed of a flexible material is partially raised to be partitioned it into plural compartments (10), and cells are cultured in each compartment (10). In due time, the compartments (10) are removed to expand a culture area in the container. As a result, the cell density at the time of culture can be maintained at an appropriate level, and an operation of transferring cells from one culture container to another culture container at the time of proliferating cells in a large amount can be eliminated, whereby damage on cells and risk of contamination can be reduced.
Claims16
A method of culturing cells in which a culture medium is supplied together with cells to a container formed of a flexible material, thereby to conduct cell culture, wherein,
- a bottom surface of the container is partially raised to be partitioned into a plurality of compartments,
- the cells are cultured in each compartment, and
- in due time, the compartments are removed to expand a culture area in the container.
A cell culture apparatus for conducting cell culture in which a culture medium is supplied together with cells to a container formed of a flexible material, wherein,
- a base having a flat mounting surface that holds the container is provided,
- partition pieces that can be protruded from the mounting surface for a prescribed height are embedded in the base, and
- when the partition pieces are accommodated into the base, upper end surfaces of the partition pieces are flushed with the mounting surface.
Dependencies are inferred from the claim text.