Sandwich assay design for small molecules
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsYi Feng Zheng, Tie Q. Wei, Manoj Sharma
Life of this patent
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Abstract
Methods are disclosed of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000. The method comprises preparing a first antibody that binds to the small molecule, and preparing a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.
Claims20
A method of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000, the method comprising:
- (a) preparing a first antibody that binds to the small molecule, and
- (b) preparing a second antibody that binds to the small molecule in a portion of the small molecule other than a portion to which the first antibody binds, wherein the second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule.
A method of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000, the method comprising:
- (a) preparing a first monoclonal antibody that binds to a portion of the small molecule, and
- (b) preparing a second monoclonal antibody that binds to the small molecule at a portion of the small molecule other than the portion to which the first monoclonal antibody binds, wherein the second monoclonal antibody is prepared from an immunogen that comprises the small molecule that is modified at the portion of the small molecule to which the first monoclonal antibody binds.
A method of designing antibodies for a sandwich assay for sirolimus, the method comprising:
- (a) preparing a first monoclonal antibody that binds to sirolimus, and
- (b) preparing a second monoclonal antibody that binds to sirolimus in a portion of sirolimus other than the portion to which the first monoclonal antibody binds, wherein the second monoclonal antibody is prepared from an immunogen that is modified at the portion of sirolimus to which the first antibody binds.
Dependencies are inferred from the claim text.