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US 2017/0362282 A1Published application
Engineered programmable molecular scaffolds from porous protein crystals
AssigneeColorado State University Research Foundation
InventorsChristopher D. Snow, Thaddaus R. Huber
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Abstract
The present disclosure provides compositions and methods for preparing engineered porous protein crystals comprising at least one guest molecule.
Claims35
A composition comprising an engineered porous protein crystal and at least one guest molecule,
- wherein the engineered porous protein crystal has a pore size equal to or greater than 3 nm and
- wherein the at least one guest molecule comprises a nanoparticle, a biomacromolecule, or a combination thereof.
A method for preparing a porous protein crystal guest molecule conjugate, the method comprising:
- obtaining a porous protein crystal, wherein the porous protein crystal has been reacted with a crosslinking agent to produce a crosslinked porous protein crystal and the crosslinking agent bonds adjacent monomers of the porous protein crystal;
- and
- incubating the crosslinked porous protein crystal with at least one guest molecule to produce a porous protein crystal guest molecule conjugate.
A method for determine the molecular structure of at least one guest molecule conjugated to a porous protein crystal, the method comprising:
- (a) obtaining a porous protein crystal guest molecule conjugate, wherein the porous protein crystal guest molecule conjugate comprises a guest molecule and a porous protein crystal, wherein the porous protein crystal has been reacted with a crosslinking agent to produce a crosslinked porous protein crystal and the crosslinking agent crosslinks adjacent monomers of the porous protein crystal;
- and
- (b) imaging the porous protein crystal guest molecule conjugate to determine the molecular structure of the at least one guest molecule.
Dependencies are inferred from the claim text.