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US 2017/0362278 A1Published application
Mammalian cells expressing cytomegalovirus antigens
AssigneeGlaxosmithkline Biologicals SA
InventorsAndrea CARFI, Claudio CIFERRI, Irmgard HOFMANN, Holger LAUX, Anders LILJA, Yingxia WEN
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Abstract
This invention relates to cytomegalovirus (CMV) proteins suitable for vaccine uses. Provided herein are mammalian host cells, in particular CHO cells, in which the sequence(s) encoding CMV proteins gH, gL, pUL128, pUL130, pUL131 (or a complex-forming fragment thereof) are stably integrated into the genome.
Claims19
1-15. (canceled)
A recombinant CHO cell, comprising:
- a polynucleotide sequence encoding cytomegalovirus (CMV) pentameric complex, wherein said pentameric complex comprises:
- gH or a complex-forming fragment thereof, gL or a complex-forming fragment thereof, pUL128 or a complex-forming fragment thereof, pUL130 or a complex-forming fragment thereof, and pUL131 or a complex-forming fragment thereof;
- wherein said one or more polynucleotide sequences are integrated into the genomic DNA of said CHO cell.
The large scale culture of 30, wherein the yield of said pentameric complex is at least 0.05 g/L.
Dependencies are inferred from the claim text.