Luterion and separating and culturing methods for same
AssigneeLuterion Co., Ltd.
InventorsYoung Ah Kwon, Won Cheol Choi, Suk Hoon Choi, Chang Hoon Choi
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to luterion, which is a mitochondrial-like micro-material, and separating and culturing methods for same and, more particularly, to a method for separating luterion by means of a filter having a pore of a particular size, luterion which is separated by means of the method thereof and has unique properties, and a culturing method for proliferation of the luterion.
Claims25
-2. (canceled)
A method for isolating the luterion comprising the steps of:
- (a) filtering the condensate obtained by cooling the water vapor or gas obtained from boiling the extract of the plant or food with a filter having a pore size of 0.8 μm to 1.2 μm;
- (b) centrifuging the filtered condensate;
- and
- (c) separating the luterion from the centrifuged supernatant.
(canceled)
A method of isolating the luterion, comprising the steps of:
- (a) adding a particle to an extract comprising the luterion so as to induce combination of the particle and the luterion, the particle having a fixed antibody or aptamer therein, which specifically binds to the luterion surface antigen;
- and (b) recovering the luterion bound to the particle.
(canceled)
-31. (canceled)
A method for culturing the luterion having at least one characteristic selected from the following, comprising adding water to the luterion and proliferating at 18° C. to 30° C. under IR light irradiation or under pressure;
- (a) in a circular or oval shape with a size of 50 nm to 800 nm and having motility;
- (b) having a nucleic acid;
- (c) showing a similar reaction as mitochondria upon immunochemical fluorescence staining;
- (d) indicating fusion and/or fission biological phenomena;
- (e) maturing to a size of up to 500 nm in the absence of fusion, maturing into pseudo-mitochondria containing DNA, and showing a mitochondria-like structure on an image obtained by a scanning electron microscope (SEM) or transmission electron microscope (TEM);
- (f) showing light reaction different from the exosome;
- (g) undergoing fission upon IR irradiation or pressurization;
- (h) expressing CD332, CD133, CD73 or CD39 as a surface antigen;
- (i) exhibiting autofluorescence;
- (j) producing ATP at a size of 200 nm to 400 nm;
- (k) having a double membrane or multi membrane structure;
- (l) showing adherence;
- (m) inhibiting telomerase activity of cancer cells;
- (n) promoting telomerase activity of normal cells;
- (o) showing cell permeability;
- and
- (p) showing blood-brain-barrier (BBB) permeability.
A method for culturing the luterion having at least one characteristic selected from the following, comprising proliferating the luterion in a sugar-containing medium at pH 5-9 and at 18° C. to 30° C.;
- (a) in a circular or oval shape with a size of 50 nm to 800 nm and having motility;
- (b) having a nucleic acid;
- (c) showing a similar reaction as mitochondria upon immunochemical fluorescence staining;
- (d) indicating fusion and/or fission biological phenomena;
- (e) maturing to a size of up to 500 nm in the absence of fusion, maturing into pseudo-mitochondria containing DNA, and showing a mitochondria-like structure on an image obtained by a scanning electron microscope (SEM) or transmission electron microscope (TEM);
- (f) showing light reaction different from the exosome;
- (g) undergoing fission upon IR irradiation or pressurization;
- (h) expressing CD332, CD133, CD73 or CD39 as a surface antigen;
- (i) exhibiting autofluorescence;
- (j) producing ATP at a size of 200 nm to 400 nm;
- (k) having a double membrane or multi membrane structure;
- (l) showing adherence;
- (m) inhibiting telomerase activity of cancer cells;
- (n) promoting telomerase activity of normal cells;
- (o) showing cell permeability;
- and
- (p) showing blood-brain-barrier (BBB) permeability.
-35. (canceled)
Dependencies are inferred from the claim text.