Method for producing the laminate of a sheet-shaped cell culture and fibrin gels

A method for treating a disease associated with tissue abnormality in a subject, comprising:

• administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture, and wherein the laminate is produced by a method comprising:
• forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein:
• the cell culture is prepared on a culture dish;
• the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart;
• and
• suturing the laminate of the fibrin gel and the cell culture to the tissue abnormality in the subject.

A method for treating a disease associated with tissue abnormality in a subject, comprising:

• administering an amount of a pharmaceutical composition to a subject in need of treating a disease associated with tissue abnormality, the pharmaceutical composition comprising a laminate comprising a fibrin gel and a cell culture, and wherein the laminate is produced by a method comprising:
• forming a fibrin gel layer on an upper surface of the cell culture by a reaction between fibrinogen and thrombin, wherein:
• the cell culture is prepared on a culture dish;
• and
• the laminate of the fibrin gel and the cell culture are transferred onto a subject's heart;
• and
• placing a side on which the fibrin gel is not formed of the laminate of the fibrin gel and the cell culture on a surface of a subject's heart.