Modifying bacteriophage
AssigneePhico Therapeutics Ltd.
InventorsHeather FAIRHEAD, Adam WILKINSON, Anne BARNARD, Emmanuele SEVERI, Neil ANDERSON, Katy PITTS
Life of this patent
Estimating the term…
Abstract
A method for modifying the genome of a lytic target phage, uses of the method and products thereof are described. Compositions comprising such phage are also described. The compositions may be formulated as a medicament, which are useful for human treatment and may treat various conditions, including bacterial infections.
Claims21
1-35. canceled
A method for modifying the genome of a lytic target phage, wherein the target phage genome includes a first target sequence and a second target sequence, which method comprises:
- (a) providing a vector which contains a phage-targeting region comprising a host range determinant of a marker phage, different from the host range determinant of the target phage, wherein the phage-targeting region is flanked by first and second flanking sequences homologous to the first and second target sequences of the target phage genome;
- (b) introducing the vector into a first host cell, which host cell is a host for the target phage;
- (c) infecting the first host cell with the target phage;
- (d) allowing replication and recombination of phage to take place whereby the genome of the target phage is modified;
- (e) propagating resultant phage on a second host cell, which host cell is a host for the marker phage and not the target phage;
- and
- (f) harvesting the resultant phage.
A method for modifying the genome of a lytic target phage, wherein the target phage genome includes a first target sequence and a second target sequence, which method comprises:
- (a) providing a vector which contains a phage-targeting region comprising a host range determinant of a marker phage, different from the host range determinant of the target phage, wherein the phage-targeting region is flanked by first and second flanking sequences homologous to the first and second target sequences of the target phage genome;
- (b) introducing the vector into a first host cell, which host cell is a host for the target phage;
- (c) infecting the first host cell with the target phage;
- (d) allowing replication and recombination of phage to take place whereby the genome of the target phage is modified;
- (e) propagating resultant phage on a second host cell, which host cell is a host for the marker phage and not the target phage;
- and
- (f) harvesting the resultant phage,
- wherein the phage-targeting region of the vector further comprises an exogenous DNA sequence for incorporation into the genome of the target phage.
A method for modifying the genome of a lytic target phage, wherein the target phage genome includes a first target sequence and a second target sequence, which method comprises:
- (a) providing a vector which contains a phage-targeting region comprising a host range determinant of a marker phage, different from the host range determinant of the target phage, wherein the phage-targeting region is flanked by first and second flanking sequences homologous to the first and second target sequences of the target phage genome;
- (b) introducing the vector into a first host cell, which host cell is a host for the target phage;
- (c) infecting the first host cell with the target phage;
- (d) allowing replication and recombination of phage to take place whereby the genome of the target phage is modified;
- (e) propagating resultant phage on a second host cell, which host cell is a host for the marker phage and not the target phage;
- and
- (f) harvesting the resultant phage,
- wherein at least one of the first and second flanking sequences contains a mutation or a point mutation as compared with the first and second target sequences of the target phage genome.
Dependencies are inferred from the claim text.