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US 2017/0304324 A1Published application
Method for obtaining vitamin d2 from shiitake mushrooms
AssigneeJangheung County Research Institute for Mushroom Industry
InventorsKyung-Je KIM, Kyoung Sun SEO, Tae-Young PARK, Seong-Woo JIN, Bong-Suk CHOI, Jin-Kyeong KIM
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Abstract
A method of obtaining vitamin D from shiitake includes a) adding ethanol to shiitake powder, followed by reflux extraction, to prepare a shiitake extract; and (b) irradiating ultraviolet (UV) light to the shiitake extract prepared in step (a). When shiitake extracts are obtained and exposed to ultraviolet light under specific conditions, the content of vitamin D2 in the shiitake extracts may be increased.
Claims4
1:
- A method of obtaining vitamin D from shiitake, the method comprising:
- adding ethanol to shiitake powder, followed by reflux extraction, to prepare a shiitake extract;
- and
- irradiating ultraviolet (UV) light to the prepared shiitake extract.
1 more element
2:
- The method of claim 1, wherein the reflux extraction is performed at a temperature of 75° C. to 85° C. for 1 to 3 hours after 90 to 100% (v/v) of ethanol is added to the shiitake powder.
3:
- The method of claim 1, wherein the UV light is irradiated to the shiitake extract by using three to five UV lamps for 3 minutes or one UV lamp for 16 minutes to 20 minutes, each UV lamp transmitting UV light having the intensity of 24 μW/cm2 to 28 μW/cm2.
4:
- The method of claim 1, wherein 90 to 100% (v/v) of the ethanol;
- the shiitake powder to which the ethanol is added is powder of pileus of the shiitake;
- the reflux extraction is performed at a temperature of 75° C. to 85° C. for 1 hour to 3 hours;
- and
- the irradiating comprises using three to five UV lamps for 3 minutes or one UV lamp for 16 minutes to 20 minutes, each UV lamp transmitting UV light having the intensity of 24 μW/cm2 to 28 μW/cm2.
2 more elements