Back to results
US 2017/0304212 A1Published application
Gene carrier using cell-derived nanovesicles and method for preparing the same
AssigneeUniversity-Industry Foundation, Yonsei University
InventorsSeung-Woo CHO, Yoon Hee JIN
Life of this patent
Estimating the term…
Abstract
Provided are a gene carrier using cell-derived nanovesicles and a method for preparing the same. The gene carrier prepared by inserting a gene into the nanovesicles artificially outbudded from a plasma membrane has excellent delivery efficiency to a target organ and cells, induces long-term regulation of gene expression, and facilitates mass production due to a simple preparation process, and thus can be used as a core technique for the gene or cell therapeutic agent field.
Claims14
A method for preparing a gene carrier, comprising:
- (a) preparing nanovesicles by extruding a suspension containing vesicles outbudded from cells;
- and
- (b) inserting a nucleotide into the prepared nanovesicles,
- wherein the vesicles are outbudded by being cultured in a buffer solution containing paraformaldehyde (PFA) and dithiothreitol (DTT), and
- the nucleotide is selected from the group consisting of mRNA, tRNA, rRNA, siRNA, and miRNA.
2 more elements
A method for preparing a gene carrier, comprising:
- (a) preparing microvesicles by centrifuging a suspension containing vesicles outbudded from cells;
- (b) treating the microvesicles with a surfactant and a nucleotide to insert the nucleotide in the microvesicles;
- and
- (c) preparing nanovesicles by extruding a suspension containing the nucleotide-inserted microvesicles,
- wherein the vesicles are outbudded by being cultured in a buffer solution containing paraformaldehyde (PFA) and dithiothreitol (DTT), and
- the nucleotide is selected from the group consisting of gDNA, pDNA, and cDNA.
3 more elements
Dependencies are inferred from the claim text.