Fluorescence assay for intestinal permeability
AssigneeThe Regents of the University of California
InventorsBakthan SINGARAM, Angel RESENDEZ, Dominic Luc WEBB
Life of this patent
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Abstract
The present disclosure relates to a method for quantifying sugar appearing in a biological fluid of a subject, the method comprising: (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Disclosed are also assays and kits for performing the above methods.
Claims22
A method for quantifying a sugar appearing in a biological sample of a subject, the method comprising:
- (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after ingestion;
- and
- (b) measuring the amount of the one or more sugars in the biological sample with an organoboronic compound coupled to a fluorophore.
-22:
- (canceled)
An assay for quantifying one or more sugars in a biological sample, the assay comprising:
- (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting;
- (b) passing the biological sample through a solid phase extraction column;
- (c) putting an amount of the biological sample into a well of one or more microtiter plates, the well of the microtiter plate further comprising a solution of an organoboronic compound, a fluorophore, and a buffer;
- (d) centrifuging the one or more microtiter plates containing the biological sample;
- (e) measuring the fluorescence of the biological sample solution in the well of the one or more microtiter plates;
- and
- (f) determining the amount of one or more sugars in the biological sample from the fluorescence in the sample.
25-27:
- (canceled)
A method of screening an adverse effect of a compound in small intestine of a subject for, the method comprising:
- (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting;
- (b) obtaining a second biological sample from the subject, who has ingested a new drug and one or more sugars, over a period of 0 to 24 hours after the ingesting;
- (c) measuring the amount of the one or more sugars in the two biological samples with an organoboronic compound coupled to a fluorophore;
- and
- (d) comparing the amount of the one or more sugars in the two biological samples to determine if the new drug resulted in increased or decreased small intestinal permeability.
Dependencies are inferred from the claim text.