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US 2017/0285009 A1Published application
Methods for identifying a receptor for a ligand and uses thereof
AssigneeUniversite De Geneve
InventorsIVAN RODRIGUEZ, ALAN JACQUES HENRI CYRUS CARLETON, DANIEL ROSSIER
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Abstract
The present invention relates to a novel method for identifying pairs of receptors/ligands, transgenic animals useful for carrying out said method, and the use of ligands and/or modulators of the interaction between a ligand and its receptor in the food industry, fragrance industry, and health industry, for instance.
Claims21
1-20. (canceled)
A method of identifying at least one chemoreceptor responding at least one ligand comprising the steps of:
- a) providing a biological sample comprising cells expressing at least one chemoreceptor, wherein said biological sample (i) has been exposed to at least one test compound or (ii) was obtained from an animal that has been exposed to at least one test compound;
- b) measuring a signal that is proportional to the level of transcription of a gene encoding at least one chemoreceptor in said biological sample, and
- c) comparing the level of signal determined in step b) to the level of signal determined in the same conditions with a negative control where the biological sample or animal has not been exposed to said at least one test compound;
- wherein a difference between the level of signal determined in step b) and the level of signal determined in the same conditions with a negative control indicates that said at least one test compound constitutes a ligand for said at least one chemoreceptor and is able to bind and modulate the activity of said at least one chemoreceptor.
1 more element
A transgenic non-human animal expressing at least 5 exogenous chemoreceptor genes.
Dependencies are inferred from the claim text.