Test strip and method of operation thereof

A test strip for biomaterials, comprising:

• a substrate, wherein the substrate is a layer of polyester;
• an electrode set disposed on the substrate, wherein the electrode set comprises a first electrode, a second electrode, and the third electrode;
• an insulating layer disposed on the electrode set, wherein the insulating layer comprises a track at one end of the insulating layer;
• a cover disposed on the insulating layer, wherein the cover comprises an inlet, an indication line, and at least one vent;
• wherein the first electrode contains a first reaction region exposed to the track;
• wherein the third electrode contains a third reaction region exposed to the track;
• wherein the second electrode contains a second reaction region;
• wherein the first reaction region is configured at the outer area of the track, the third reaction region is configured at the inner area of the track, and the second reaction region is configured between the first reaction region and the third reaction region;
• wherein the inlet is disposed at one end of the cover close to the track;
• wherein the indication line is configured at one end of the track away from the inlet;
• and
• wherein the at least one vent is configured with the indication line away from the inlet.