Method for calibrating fluorescent plate readers
AssigneePlexbio Co., Ltd.
InventorsCheng-Tse LIN, Yi-Ming SUN, Kuei-Shen HSU, Liang-Han CHANG, Yao-Kuang CHUNG, Chin-Yun WU, Chin-Shiou HUANG
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Abstract
The present invention relates to a fluorescent polymer cast on the surface of wells of a plate and a plate comprising the fluorescent polymer. The plate is used in a method for calibrating read-out values of plate readers. The method for calibrating the plate readers can effectively reduce the deviation of read-out values among different plate readers.
Claims22
A fluorescent polymer comprising a pre-determined amount of a fluorescent dye imbedded in a polymer, wherein the fluorescent polymer is transparent, dry and solvent- and porogen-free.
A method of producing a fluorescent polymer comprising:
- mixing a monomer and a pre-determined amount of fluorescent dye;
- and forming the fluorescent polymer by polymerizing the monomer with a polymerization initiator, wherein the fluorescent polymer is transparent, dry and solvent- and porogen-free;
- provided that no liquid solvent is added in the method.
A method of producing a fluorescent polymer plate, comprising:
- (a) mixing a monomer solution and a pre-determined amount of fluorescent dye;
- (b) adding the resulting mixture of (a) to wells of the plate;
- and polymerizing the monomer with a polymerization initiator on the wells, wherein the fluorescent polymer is transparent, dry and solvent- and porogen-free;
- provided that no liquid solvent is added in the method.
Dependencies are inferred from the claim text.