Two-color fluorescence localization super-resolution biological microscopy method and system

A two-color fluorescence localized super-resolution biological microscopy system, comprising:

• A sample labeling module for performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution;
• a signal generation module for illuminating the biological sample by laser to generate a blinking fluorescent signal of a first channel corresponding to the Alexa647 or Cy5 fluorescent molecules as well as a blinking fluorescent signal of a second channel corresponding to the Alexa750 or Cy7 fluorescent molecules respectively;
• a first image construction module for constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively according to the blinking fluorescent signal of the first channel and the blinking fluorescent signal of the second channel;
• and
• a second image construction module for aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure to construct a super-resolution image of a third biological structure.