US 2017/0284908 A1 · AUTOSTAINER HEMATOXYLIN AND METHODS OF USE

Abstract

This disclosure relates to hematoxylin staining formulations and particularly to formulations for use in autostainers. The disclosed compositions were discovered to possess atypical stability under storage while having high stain quality and sufficiently fast staining performance. The disclosed hematoxylin staining compositions include a solvent system, hematoxylin, a chemical oxidant, and a mordant. Illustrative embodiments also have a Cl−/SO4− molar ratio of between about 2.5/1 and about 1/4.

Claims36

Dependent claims

A hematoxylin staining composition comprising:

a solvent, hematoxylin, an amount of a chemical oxidant sufficient to convert at least a portion of the hematoxylin to hematein, a mordant, wherein the composition further comprises chloride (Cl−) and sulfate (SO42−), wherein the chloride/sulfate molar ratio is between about 2.5/1 and about 1/4.

Dependencies are inferred from the claim text.

Facts

Application

15/627,183Filed 19 Jun 2017

Priority

18 Dec 2014

Published

5 Oct 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 1/30
Preparing specimens for investigation including physical details of chemical methods covered elsewhere, e.g. G01N33/50, C12Q: staining;…
G01N 1/312
Apparatus therefor: for samples mounted on planar substrates
G01N 21/253
Colorimeters; Construction thereof: for batch operation, i.e. multisample apparatus
G01N 2001/302
Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like…:…

Autostainer hematoxylin and methods of use

Life of this patent

Autostainer hematoxylin and methods of use

Life of this patent

Abstract

Claims36