US 2017/0283887 A1Published application

Diagnosis of lung cancer

AssigneeAit Austrian Institute of Technology GmbH

InventorsMatthias Wielscher, Andreas Weinhäusel, Klemens Vierlinger, Christa Noehammer, Rolf Ziesche, Andrea Gsur

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Abstract

The present invention relates to specifically methylated nucleic acids, methods of diagnosing lung cancer or a predisposition therefor, and a corresponding kit.

Claims15

Dependent claims

A nucleic acid comprising

(a) the sequence
(1) from position 7 to position 101 of SEQ ID NO:
4, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(2) from position 11 to position 71 of SEQ ID NO:
2, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(3) from position 37 to position 81 of SEQ ID NO:
3, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(4) from position 4 to position 84 of SEQ ID NO:
1, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(5) from position 28 to position 69 of SEQ ID NO:
5, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(6) from position 39 to position 85 of SEQ ID NO:
6, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(7) from position 10 to position 53 of SEQ ID NO:
7, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(8) from position 13 to position 71 of SEQ ID NO:
8, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(9) from position 5 to position 71 of SEQ ID NO:
9, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(10) from position 2 to position 60 of SEQ ID NO:
10, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(11) from position 22 to position 63 of SEQ ID NO:
11, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(12) from position 45 to position 67 of SEQ ID NO:
12, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(13) from position 58 to position 78 of SEQ ID NO:
13, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(14) from position 31 to position 66 of SEQ ID NO:
14, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(15) from position 41 to position 72 of SEQ ID NO:
15, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(16) from position 53 to position 82 of SEQ ID NO:
16, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(17) from position 20 to position 74 of SEQ ID NO:
17, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(18) from position 9 to position 98 of SEQ ID NO:
18, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(19) from position 23 to position 85 of SEQ ID NO:
19, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(20) from position 20 to position 28 of SEQ ID NO:
20, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(21) from position 24 to position 61 of SEQ ID NO:
21, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(22) from position 11 to position 84 of SEQ ID NO:
22, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(23) from position 2 to position 86 of SEQ ID NO:
23, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(24) from position 43 to position 80 of SEQ ID NO:
24, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(25) from position 24 to position 70 of SEQ ID NO:
25, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(26) from position 44 to position 94 of SEQ ID NO:
26, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(27) from position 44 to position 67 of SEQ ID NO:
27, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(28) from position 20 to position 43 of SEQ ID NO:
28, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(29) from position 3 to position 71 of SEQ ID NO:
29, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(30) from position 11 to position 64 of SEQ ID NO:
30, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(31) from position 7 to position 118 of SEQ ID NO:
31, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(32) from position 25 to position 95 of SEQ ID NO:
32, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(33) from position 29 to position 46 of SEQ ID NO:
33, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(34) from position 8 to position 72 of SEQ ID NO:
34, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(35) from position 5 to position 57 of SEQ ID NO:
35, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(36) from position 43 to position 67 of SEQ ID NO:
36, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(37) from position 21 to position 63 of SEQ ID NO:
37, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(38) from position 27 to position 86 of SEQ ID NO:
38, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(39) from position 2 to position 110 of SEQ ID NO:
39, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(40) from position 13 to position 59 of SEQ ID NO:
40, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(41) from position 32 to position 107 of SEQ ID NO:
41, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(42) from position 3 to position 48 of SEQ ID NO:
42, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(43) from position 5 to position 57 of SEQ ID NO:
43, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(44) from position 40 to position 106 of SEQ ID NO:
44, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(45) from position 26 to position 90 of SEQ ID NO:
45, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
or
(46) from position 68 to position 74 of SEQ ID NO:
46, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
or
(b) a sequence heaving at least 80% sequence identity to a sequence of (a).

93 more elements

A method of diagnosing lung cancer or a predisposition therefor, said method comprising determining the methylation status of cytidine in

(1) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 7 to position 101 of SEQ ID NO:
4;
(2) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 11 to position 71 of SEQ ID NO:
2;
(3) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 37 to position 81 of SEQ ID NO:
3;
(4) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 4 to position 84 of SEQ ID NO:
1;
(5) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 28 to position 69 of SEQ ID NO:
5;
(6) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 39 to position 85 of SEQ ID NO:
6;
(7) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 10 to position 53 of SEQ ID NO:
7;
(8) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 13 to position 71 of SEQ ID NO:
8;
(9) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 5 to position 71 of SEQ ID NO:
9;
(10) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 2 to position 60 of SEQ ID NO:
10;
(11) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 22 to position 63 of SEQ ID NO:
11;
(12) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 45 to position 67 of SEQ ID NO:
12;
(13) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 58 to position 78 of SEQ ID NO:
13;
(14) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 31 to position 66 of SEQ ID NO:
14;
(15) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 41 to position 72 of SEQ ID NO:
15;
(16) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 53 to position 82 of SEQ ID NO:
16;
(17) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 20 to position 74 of SEQ ID NO:
17;
(18) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 9 to position 98 of SEQ ID NO:
18;
(19) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 23 to position 85 of SEQ ID NO:
19;
(20) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 20 to position 28 of SEQ ID NO:
20;
(21) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 24 to position 61 of SEQ ID NO:
21;
(22) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 11 to position 84 of SEQ ID NO:
22;
(23) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 2 to position 86 of SEQ ID NO:
23;
(24) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 43 to position 80 of SEQ ID NO:
24;
(25) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 24 to position 70 of SEQ ID NO:
25;
(26) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 44 to position 94 of SEQ ID NO:
26;
(27) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 44 to position 67 of SEQ ID NO:
27;
(28) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 20 to position 43 of SEQ ID NO:
28;
(29) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 3 to position 71 of SEQ ID NO:
29;
(30) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 11 to position 64 of SEQ ID NO:
30;
(31) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 7 to position 118 of SEQ ID NO:
31;
(32) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 25 to position 95 of SEQ ID NO:
32;
(33) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 29 to position 46 of SEQ ID NO:
33;
(34) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 8 to position 72 of SEQ ID NO:
34;
(35) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 5 to position 57 of SEQ ID NO:
35;
(36) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 43 to position 67 of SEQ ID NO:
36;
(37) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 21 to position 63 of SEQ ID NO:
37;
(38) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 27 to position 86 of SEQ ID NO:
38;
(39) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 2 to position 110 of SEQ ID NO:
39;
(40) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 13 to position 59 of SEQ ID NO:
40;
(41) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 32 to position 107 of SEQ ID NO:
41;
(42) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 3 to position 48 of SEQ ID NO:
42;
(43) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 5 to position 57 of SEQ ID NO:
43;
(44) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 40 to position 106 of SEQ ID NO:
44;
(45) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 26 to position 90 of SEQ ID NO:
45;
and/or
(46) at least one CpG dinucleotide in a nucleic acid comprising or consisting of the sequence from position 68 to position 74 of SEQ ID NO:
46;
wherein methylation of the cytidine(s) or increased methylation of the cytidine(s) as compared to a non-cancer subject is indicative of lung cancer or a predisposition therefor, and wherein any diagnostic method practiced on the human or animal body is excluded.

91 more elements

A kit comprising one or more of the following sets of primers:

(1) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
229 and 230 or sequences having at least 80% sequence identity thereto;
(2) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
231 and 232 or sequences having at least 80% sequence identity thereto;
(3) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
233 and 234 or sequences having at least 80% sequence identity thereto;
(4) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
235 and 236 or sequences having at least 80% sequence identity thereto;
(5) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
237 and 238 or sequences having at least 80% sequence identity thereto;
(6) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
239 and 240 or sequences having at least 80% sequence identity thereto;
(7) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
241 and 242 or sequences having at least 80% sequence identity thereto;
(8) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
243 and 244 or sequences having at least 80% sequence identity thereto;
(9) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
245 and 246 or sequences having at least 80% sequence identity thereto;
(10) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
247 and 248 or sequences having at least 80% sequence identity thereto;
(11) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
249 and 250 or sequences having at least 80% sequence identity thereto;
(12) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
251 and 252 or sequences having at least 80% sequence identity thereto;
(13) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
253 and 254 or sequences having at least 80% sequence identity thereto;
(14) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
255 and 256 or sequences having at least 80% sequence identity thereto;
(15) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
257 and 258 or sequences having at least 80% sequence identity thereto;
(16) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
259 and 260 or sequences having at least 80% sequence identity thereto;
(17) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
261 and 262 or sequences having at least 80% sequence identity thereto;
(18) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
263 and 264 or sequences having at least 80% sequence identity thereto;
(19) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
265 and 266 or sequences having at least 80% sequence identity thereto;
(20) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
267 and 268 or sequences having at least 80% sequence identity thereto;
(21) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
269 and 270 or sequences having at least 80% sequence identity thereto;
(22) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
271 and 272 or sequences having at least 80% sequence identity thereto;
(23) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
273 and 274 or sequences having at least 80% sequence identity thereto;
(24) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
275 and 276 or sequences having at least 80% sequence identity thereto;
(25) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
277 and 278 or sequences having at least 80% sequence identity thereto;
(26) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
279 and 280 or sequences having at least 80% sequence identity thereto;
(27) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
281 and 282 or sequences having at least 80% sequence identity thereto;
(28) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
283 and 284 or sequences having at least 80% sequence identity thereto;
(29) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
285 and 286 or sequences having at least 80% sequence identity thereto;
(30) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
287 and 288 or sequences having at least 80% sequence identity thereto;
(31) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
289 and 290 or sequences having at least 80% sequence identity thereto;
(32) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
291 and 292 or sequences having at least 80% sequence identity thereto;
(33) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
293 and 294 or sequences having at least 80% sequence identity thereto;
(34) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
295 and 296 or sequences having at least 80% sequence identity thereto;
(35) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
297 and 298 or sequences having at least 80% sequence identity thereto;
(36) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
299 and 300 or sequences having at least 80% sequence identity thereto;
(37) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
301 and 302 or sequences having at least 80% sequence identity thereto;
(38) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
303 and 304 or sequences having at least 80% sequence identity thereto;
(39) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
305 and 306 or sequences having at least 80% sequence identity thereto;
(40) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
307 and 308 or sequences having at least 80% sequence identity thereto;
(41) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
309 and 310 or sequences having at least 80% sequence identity thereto;
(42) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
311 and 312 or sequences having at least 80% sequence identity thereto;
(43) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
313 and 314 or sequences having at least 80% sequence identity thereto;
(44) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
315 and 316 or sequences having at least 80% sequence identity thereto;
(45) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
317 and 318 or sequences having at least 80% sequence identity thereto;
(46) nucleic acids comprising or consisting of the sequences of SEQ ID NO:
319 and 320 or sequences having at least 80% sequence identity thereto.

89 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/529,338Filed 24 Nov 2015
Priority: EP 14194732.525 Nov 2014
Published: 5 Oct 2017
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6886
For diseases caused by alterations of genetic material: for cancer
C12Q 2600/118
Oligonucleotides characterized by their use: prognosis of disease development
C12Q 2600/154
Oligonucleotides characterized by their use: methylation markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

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Abstract

The present invention relates to specifically methylated nucleic acids, methods of diagnosing lung cancer or a predisposition therefor, and a corresponding kit.

A nucleic acid comprising

(a) the sequence
(1) from position 7 to position 101 of SEQ ID NO:
4, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(2) from position 11 to position 71 of SEQ ID NO:
2, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(3) from position 37 to position 81 of SEQ ID NO:
3, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(4) from position 4 to position 84 of SEQ ID NO:
1, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(5) from position 28 to position 69 of SEQ ID NO:
5, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(6) from position 39 to position 85 of SEQ ID NO:
6, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(7) from position 10 to position 53 of SEQ ID NO:
7, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(8) from position 13 to position 71 of SEQ ID NO:
8, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(9) from position 5 to position 71 of SEQ ID NO:
9, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(10) from position 2 to position 60 of SEQ ID NO:
10, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(11) from position 22 to position 63 of SEQ ID NO:
11, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(12) from position 45 to position 67 of SEQ ID NO:
12, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(13) from position 58 to position 78 of SEQ ID NO:
13, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(14) from position 31 to position 66 of SEQ ID NO:
14, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(15) from position 41 to position 72 of SEQ ID NO:
15, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(16) from position 53 to position 82 of SEQ ID NO:
16, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(17) from position 20 to position 74 of SEQ ID NO:
17, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(18) from position 9 to position 98 of SEQ ID NO:
18, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(19) from position 23 to position 85 of SEQ ID NO:
19, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(20) from position 20 to position 28 of SEQ ID NO:
20, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(21) from position 24 to position 61 of SEQ ID NO:
21, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(22) from position 11 to position 84 of SEQ ID NO:
22, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(23) from position 2 to position 86 of SEQ ID NO:
23, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(24) from position 43 to position 80 of SEQ ID NO:
24, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(25) from position 24 to position 70 of SEQ ID NO:
25, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(26) from position 44 to position 94 of SEQ ID NO:
26, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(27) from position 44 to position 67 of SEQ ID NO:
27, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(28) from position 20 to position 43 of SEQ ID NO:
28, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(29) from position 3 to position 71 of SEQ ID NO:
29, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(30) from position 11 to position 64 of SEQ ID NO:
30, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(31) from position 7 to position 118 of SEQ ID NO:
31, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(32) from position 25 to position 95 of SEQ ID NO:
32, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(33) from position 29 to position 46 of SEQ ID NO:
33, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(34) from position 8 to position 72 of SEQ ID NO:
34, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(35) from position 5 to position 57 of SEQ ID NO:
35, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(36) from position 43 to position 67 of SEQ ID NO:
36, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(37) from position 21 to position 63 of SEQ ID NO:
37, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(38) from position 27 to position 86 of SEQ ID NO:
38, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(39) from position 2 to position 110 of SEQ ID NO:
39, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(40) from position 13 to position 59 of SEQ ID NO:
40, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(41) from position 32 to position 107 of SEQ ID NO:
41, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(42) from position 3 to position 48 of SEQ ID NO:
42, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(43) from position 5 to position 57 of SEQ ID NO:
43, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(44) from position 40 to position 106 of SEQ ID NO:
44, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
(45) from position 26 to position 90 of SEQ ID NO:
45, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
or
(46) from position 68 to position 74 of SEQ ID NO:
46, wherein the cytidine in at least one CpG dinucleotide comprised in said sequence is methylated;
or
(b) a sequence heaving at least 80% sequence identity to a sequence of (a).

93 more elements

Facts

Application

15/529,338Filed 24 Nov 2015

Priority

EP 14194732.525 Nov 2014

Published

5 Oct 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6886
For diseases caused by alterations of genetic material: for cancer
C12Q 2600/118
Oligonucleotides characterized by their use: prognosis of disease development
C12Q 2600/154
Oligonucleotides characterized by their use: methylation markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms