US 2017/0283872 A1 · COMPOSITIONS AND METHODS FOR ENHANCING ODORANT RECEPTOR ACTIVITY

Abstract

The present invention relates to polypeptides capable of modulating odorant receptor activation. In particular, the present invention provides polypeptides (e.g., type 3 muscarinic actetylcholine receptor M3) capable of enhancing odorant receptor activation. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for polypeptide polymorphisms and mutations associated with odorant receptor activation (e.g., polymorphisms and mutations associated with muscarinic actetylcholine receptor polypeptides (e.g., M1, M2, M3, M4, M5)), as well as methods of screening for therapeutic agents, ligands, and modulators of such proteins.

Claims24

Dependent claims

A method for identifying an odorant receptor ligand, comprising:

a) providing
i) a cell comprising an odorant receptor, wherein said cell further comprises a heterologous nucleic acid encoding a polypeptide comprising an amino acid sequence that is at least 80% identical to SEQ ID No. 3, wherein said polypeptide is capable of promoting odorant receptor activity;
ii) at least one test compound;
b) exposing said test compound to said cell;
and
c) detecting the activity of said odorant receptor.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/477,873Filed 3 Apr 2017

Priority

4 Dec 2009

Published

5 Oct 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12Q 1/6881
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for tissue or cell typing, e.g. human leukocyte antigen [HLA]…
C07K 14/70571
Receptors; Cell surface antigens; Cell surface determinants: for neuromediators, e.g. serotonin receptor, dopamine receptor
C12Q 2600/136
Oligonucleotides characterized by their use: screening for pharmacological compounds
C12Q 2600/156
Oligonucleotides characterized by their use: polymorphic or mutational markers

Family

Application61/266,805Us provisional application · filed 4 Dec 2009

Application13/513,600Continuation · filed 18 Sep 2012

Application14/754,940Continuation · filed 30 Jun 2015

Application15/477,873Continuation

US 10,072,292 B2Related patent

US 2013/0004983 A1Published application

US 2015/0376259 A1Published application

US 9,068,226 B2Related patent

US 9,611,308 B2Related patent

US 2017/0283872 A1This patent

From the US filing history. Foreign family members aren’t listed.

Compositions and methods for enhancing odorant receptor activity

Life of this patent

Compositions and methods for enhancing odorant receptor activity

Life of this patent

Abstract

Claims24