A device (2) for sampling and depositing all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a semi-solid culture medium such as an agar medium (8), and intended to be deposited into a container (9) or onto an analysis plate (14), comprising:
- a probe (3) equipped with a pointed and closed terminal end (4),
- a cooling means intended for frosting the terminal end (4),
- driving means (5) intended:
- for exerting a pressure from the probe (3) onto the sample (11) so as to freeze all or part of the water contained in the sample (11) in order to stick it to the terminal end (4),
- for separating all or part of the sample (11) from the culture medium (8),
- to bring all or part of the sample (11) to the container (9) or the analysis plate (14) and at least one contact sensor which stops the descent of the driving means (5) once contact between the terminal (4) and the biological matter (7) is made.