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US 2017/0283846 A1Published application
Articles and method for detecting listeria monocytogenes
Assignee3M Innovative Properties Company
InventorsTAKATOSHI MORIYAMA, PATRICK A. MACH, AKIO KITAHARA, HENRY J. LUBRANT
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Abstract
A method of detecting Listeria monocytogenes. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of Listeria microorganisms. When a Listeria microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect Listeria monocytogenes.
Claims14
A method for detecting an Escherichia coli microorganism in a sample, the method comprising:
- providing:
- a culture device including a culture medium comprising ingredients selected to facilitate growth of an Enterobacteriaceae microorganism;
- a detection article comprising a first indicator system, the first indicator system selected to detect Escherichia coli;
- wherein the Escherichia coli microorganism is detected by its ability to convert the first indicator system from a first state to a second state;
- a second indicator system selected to detect the Enterobacteriaceae microorganism;
- and
- a sample;
- inoculating the culture device with the sample;
- placing the second indicator system in fluid communication with the culture medium;
- incubating the inoculated culture device for a period of time sufficient to permit growth of the Enterobacteriaceae microorganism, if the Enterobacteriaceae microorganism is present in the sample;
- observing the culture device to detect at least one Enterobacteriaceae microorganism;
- wherein observing the culture device for an indication of the presence of the at least one Enterobacteriaceae microorganism comprises detecting a conversion of the second indicator system from a first state to a second state;
- contacting the culture medium of the incubated culture device with the first indicator system of the detection article only if the at least one Enterobacteriaceae microorganism is detected;
- and
- observing the article-contacted culture device to detect a conversion of the first indicator system from a first state to a second state;
- wherein the first and second indicator systems are distinct;
- wherein conversion of the first indicator system from the first state to the second state by the at least one Enterobacteriaceae microorganism indicates the at least one Enterobacteriaceae microorganism is Escherichia coli.
14 more elements
A method for detecting an Escherichia coli microorganism in a sample, the method comprising:
- providing:
- a culture device including a culture medium comprising ingredients selected to facilitate growth of a coliform microorganism;
- a detection article comprising a first indicator system, the first indicator system selected to detect Escherichia coli;
- wherein the Escherichia coli microorganism is detected by its ability to convert the first indicator system from a first state to a second state;
- a second indicator system selected to detect the coliform microorganism;
- and
- a sample;
- inoculating the culture device with the sample;
- placing the second indicator system in fluid communication with the culture medium;
- incubating the inoculated culture device for a period of time sufficient to permit growth of the coliform microorganism, if the coliform microorganism is present in the sample;
- observing the culture device to detect at least one coliform microorganism;
- wherein observing the culture device for an indication of the presence of the at least one coliform microorganism comprises detecting a conversion of the second indicator system from a first state to a second state;
- contacting the culture medium of the incubated culture device with the first indicator system of the detection article only if the at least one coliform microorganism is detected;
- and
- observing the article-contacted culture device to detect a conversion of the first indicator system from a first state to a second state;
- wherein the first and second indicator systems are distinct;
- wherein conversion of the first indicator system from the first state to the second state by the at least one coliform microorganism indicates the at least one coliform microorganism is Escherichia coli.
14 more elements
Dependencies are inferred from the claim text.