Methods of producing bacterial nanocellulose from cassava bagasse
AssigneeShanghai Zhiyi Information Technology Ltd.
InventorFeng Hong
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Abstract
Methods, compositions, systems and kits relating to processing of cassava bagasse into bacterial feedstock, such as bacterial feedstock suitable for nanocellulose production, are disclosed. Cassava bagasse may be contacted with an acid catalyst or an enzymatic catalyst to produce a hydrolysate, which can be used to form a pre-fermentation medium. Incubation of the pre-fermentation medium with a first population of microorganisms yields a supernatant enriched in reducing sugars, which may be used to form a culture medium which can be used to support growth of a second population of microorganisms to form the nanocellulose.
Claims79
A method of producing bacterial nanocellulose, the method comprising:
- contacting cassava bagasse with a catalyst to form a reaction mixture, wherein the catalyst is an acid catalyst, an enzymatic catalyst or a combination thereof to produce cassava bagasse hydrolysate;
- preparing a pre-fermentation medium comprising the cassava bagasse hydrolysate;
- inoculating the pre-fermentation medium with a first population of microorganisms to produce an inoculated pre-fermentation medium;
- incubating the inoculated pre-fermentation medium to produce an enriched supernatant;
- collecting the enriched supernatant;
- preparing a culture medium comprising the enriched supernatant;
- inoculating the culture medium with a second population of microorganisms to produce an inoculated culture medium;
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- incubating the inoculated culture medium to produce bacterial nanocellulose.
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A method of producing bacterial nanocellulose, the method comprising:
- treating at least one cassava by-product to form particles of cassava byproduct having an average particle size of about 250 μm to about 420 μm;
- preparing a pre-fermentation medium comprising the particles of cassava by-product;
- inoculating the pre-fermentation medium with a first population of microorganism to produce an inoculated pre-fermentation medium, wherein the first population of microorganisms comprise Rhizopus sp., Aspergillus niger, or both;
- incubating the inoculated pre-fermentation medium to produce an enriched supernatant;
- collecting the enriched supernatant;
- preparing a culture medium comprising the enriched supernatant;
- inoculating the culture medium with a second population of microorganisms to produce an inoculated culture medium;
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- incubating the inoculated culture medium to produce bacterial nanocellulose.
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A method of making a culture medium, the method comprising:
- contacting cassava bagasse with a catalyst to form a reaction mixture, wherein the catalyst is an acid catalyst, an enzymatic catalyst, or a combination thereof;
- subjecting the reaction mixture to conditions sufficient to hydrolyze at least a portion of the cassava bagasse to produce cassava bagasse hydrolysate;
- preparing a pre-fermentation medium comprising the cassava bagasse hydrolysate;
- inoculating the pre-fermentation medium with a population of microorganisms to produce an inoculated pre-fermentation medium, wherein the population of microorganisms comprise Rhizopus sp., Aspergillus niger, or both;
- incubating the inoculated pre-fermentation medium to produce an enriched supernatant;
- collecting the enriched supernatant;
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- preparing the culture medium with the enriched supernatant.
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Dependencies are inferred from the claim text.