High oleic acid soybean seeds
OwnerKaren A Hudsoninventor
Life of this patent
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Abstract
A genetically altered soybean plant, its parts (including seeds, cells, flowers, pollen), and its progeny produces at least one altered delta-twelve fatty acid desaturase 2 enzyme (FAD2), namely an altered FAD2-1A, an altered FAD2-1B, or both. This genetically altered soybean plant has reduced FAD2 enzymatic activity in its seeds, thereby producing higher amounts of oleic acid in its seeds than a wild-type soybean plant produces. Methods of generating this genetically altered soybean plant are provided.
Claims22
A genetically altered soybean plant and parts thereof comprising an altered delta-twelve fatty acid desaturase 2-1B enzyme (FAD2-1B) and an altered delta-twelve fatty acid desaturase 2-1A enzyme (FAD2-1A), wherein said altered FAD2-1B has reduced enzymatic activity compared to wild-type FAD2-1B, wherein said altered FAD2-1A has reduced enzymatic activity compared to wild-type FAD2-1A, and wherein the seeds of said genetically altered soybean plant contain higher amounts of oleic acid compared to the amount of oleic acid in the seeds of a wild-type soybean plant having wild-type FAD2-1B and wild-type FAD2-1A.
A method for constructing a genetically altered soybean plant having an altered delta-twelve fatty acid desaturase 2-1B enzyme (FAD2-1B) and an altered delta twelve fatty acid desaturase 2-1A enzyme (FAD2-1A) and capable of producing seeds with a fatty acid content of at least 70% oleic acid, the method comprising:
- (i) introducing a nucleic acid encoding an altered FAD2-1B enzyme having a P284S mutation into a wild-type soybean plant to provide a first genetically altered soybean plant;
- (ii) selecting said first genetically altered soybean plant that is homozygous for said altered FAD2-1B enzyme,
- (iii) introducing a nucleic acid encoding said altered FAD2-1A enzyme into a wild-type soybean plant to provide a second genetically altered soybean plant;
- wherein said altered FAD2-1A enzyme is selected from the group consisting of FAD2-1AL41F, FAD2-1AV106M, FAD2-1AS154F, FAD2-1AP163S, FAD2-1AW194STOP, FAD2-1AG204D, FAD2-1AP284L, FAD2-1AP284S, and FAD2-1AA358T;
- and
- (iv) selecting said second genetically altered soybean plant that is homozygous for said altered FAD2-1B enzyme and said altered FAD2-1A enzyme;
- thereby constructing said genetically altered soybean plant capable of producing seeds with a fatty acid content of at least 70% oleic acid.
A kit useful for the identification of SNPs in FAD2-1A or FAD2-1B genes of a soybean plant comprising a plurality of polynucleotides, optionally a restriction endonuclease, optionally a polymerase, optionally an identifying dye, and optionally instructions;
- wherein said plurality of polynucleotides are selected from group consisting of SEQ ID NOs:
- 29 and 30, SEQ ID NOs:
- 31 and 32, SEQ ID NOs:
- 25 and 28, SEQ ID NOs:
- 27 and 28, SEQ ID NOs:
- 33 and 34, SEQ ID NOs:
- 35 and 36, SEQ ID NOs:
- 37 and 40, and a combination thereof.
Dependencies are inferred from the claim text.