Ttv mirna sequences as an early marker for the future development of cancer and as a target for cancer treatment and prevention
AssigneeDeutsches Krebsforschungszentrum
InventorsHarald ZUR HAUSEN, Ethel-Michel DE VILLIERS, Angel CID-ARREGUI, Victor SARACHAGA DE BENITO
Life of this patent
Estimating the term…
Abstract
Described are TTV miRNAs and probes and primers comprising part of said TTV miRNA polynucleic acid. The use of said compounds for diagnosis of cancer or predisposition of cancer is also described.
Claims10
A TTV miRNA encoded by a torque teno virus (TTV) polynucleic acid comprising:
- (a) a nucleotide sequence depicted in Table 1, 2a or 2b;
- (b) a nucleotide sequence having at least 60% identity to the nucleotide sequence of (a) and containing the nucleotide sequence CATCCYY (with Y:
- C or T);
- or
- (c) a fragment of the nucleotide sequence of (a) or (b) and containing the nucleotide sequence CATCCYY (with Y:
- C or T);
A labelled oligonucleotide probe being capable of acting as a hybridization probe for specific detection of a nucleic acid of a certain TTV isolate and comprising 5 to 50 nucleotides complementary to a nucleotide sequence selected from
- (a) a nucleotide sequence in Table 1, Table 2a or 2b;
- (b) a nucleotide sequence having at least 60% identity to the nucleotide sequence of (a) and containing the nucleotide sequence CATCCYY (with Y:
- C or T) or CAUCCYY (with Y:
- C or U), respectively;
- (c) a fragment of the nucleotide sequence of (a) or (b) and containing the nucleotide sequence CATCCYY (with Y:
- C or T) or CAUCCYY (with Y:
- C or U), respectively;
- or
- (d) a nucleotide sequence being complementary to a nucleotide sequence of (a), (b) or (c).
A vector comprising a TTV polynucleic acid selected from:
- (a) a nucleotide sequence in Table 1, Table 2a or 2b;
- (b) a nucleotide sequence having at least 60% identity to the nucleotide sequence of (a) and containing the nucleotide sequence CATCCYY (with Y:
- C or T) or CAUCCYY (with Y:
- C or U), respectively;
- (c) a fragment of the nucleotide sequence of (a) or (b) and containing the nucleotide sequence CATCCYY (with Y:
- C or T) or CAUCCYY (with Y:
- C or U), respectively;
- or
- (d) a nucleotide sequence being complementary to a nucleotide sequence of (a), (b) or (c).
A method of diagnosing colon cancer comprising the steps of:
- (a) isolating miRNA from a patients sample;
- (b) sequencing the miRNA selected in step (a);
- and
- (c) determining if a miRNA selected from the miRNAs is complementary to a sequence shown in Table 2B is present in the sample, whereas the presence of an miRNA complementary to a sequence shown in Table 2B indicates colon cancer.
Dependencies are inferred from the claim text.