GENE EXPRESSION ANALYSIS METHOD USING TWO DIMENSIONAL cDNA LIBRARY

An analysis apparatus, comprising:

• a support where a test nucleic acid-capturing part has been two-dimensionally distributed, wherein the test nucleic acid-capturing part is on the surface of and/or within the support;
• a solution supplying unit;
• and
• an analyzing unit for conducting an analysis of a test nucleic acid,
• wherein the test nucleic acid-capturing part comprises a nucleic acid probe immobilized thereto, and the nucleic acid probe is positioned below a cell on the support;
• wherein the test nucleic acid extracted from the cell on the support by a nucleic acid extraction reagent, which is supplied by the solution supplying unit, is captured to the nucleic acid probe;
• and
• wherein a complementary strand of the captured test nucleic acid is synthesized on the support by an enzyme for the complementary strand synthesis, which is supplied by the solution supplying unit, such that a cDNA library is prepared on the support.

A device, comprising:

• a support where a test nucleic acid-capturing part has been two-dimensionally distributed, wherein the test nucleic acid-capturing part is on the surface of and/or within the support;
• and
• a solution supplying unit,
• wherein the test nucleic acid-capturing part comprises a nucleic acid probe immobilized thereto, and the nucleic acid probe is positioned below a cell on the support;
• wherein the test nucleic acid extracted from the cell on the support by a nucleic acid extraction reagent, which is supplied by the solution supplying unit, is captured to the nucleic acid probe;
• and
• wherein a complementary strand of the captured test nucleic acid is synthesized on the support by an enzyme for the complementary strand synthesis, which is supplied by the solution supplying unit, such that a cDNA library is prepared on the support.