EXTRACTION OF cfDNA FROM BIOLOGICAL SAMPLES
AssigneeCovaris, Inc.
InventorsHamid Khoja, Hans-Ulrich Thomann, James A. Laugharn, JR.
Life of this patent
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Abstract
Focused acoustic treatment of samples including a target biomolecule, such as cfDNA, may aid in the recovery of the biomolecule from a sample. cfDNA in a sample, whether chemically stabilized or not, may be linked or otherwise bound to histones or other proteins, e.g., by hydrogen bonds of histones or chaperone proteins to DNA and/or covalent crosslinks of such proteins to the DNA. Focused acoustic energy may remove or disrupt such links, aiding in isolation of the cfDNA from the sample.
Claims23
A method for processing a sample containing a biomolecule to be recovered from the sample, comprising:
- providing a sample containing cfDNA that has histones and/or other proteins bound to the cfDNA in an acoustic treatment vessel;
- exposing the sample in the vessel to focused acoustic energy to remove or disrupt bonds between the cfDNA and bound histones and/or other proteins;
- and
- extracting the cfDNA from the sample after bonds between the cfDNA and bound histones and/or other proteins have been removed or disrupted.
A method for processing a sample containing intact cells and extracellular vesicles, comprising:
- providing a sample containing intact cells and extracellular vesicles in a liquid in an acoustic treatment vessel;
- and
- exposing the sample to focused acoustic energy to lyse the intact cells without lysing the extracellular vesicles.
Dependencies are inferred from the claim text.