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US 2017/0283786 A1Published application
Method for preparing kaolin immobilized gy2b bacteria and application thereof
AssigneeSouth Cina University of Technology
InventorsPingxiao WU, YUEWU LI, ZHI DANG, NENGWU ZHU
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Abstract
Provided are a method for preparing kaolin immobilized GY2B bacteria and use thereof.
Claims10
A method for preparing immobilized GY2B bacteria, comprising the steps of:
- activating a GY2B bacteria culture:
- growing the activated GY2B bacterial culture in a phenol-containing enrichment culture solution, so as to obtain a concentrated bacterial solution centrifuging the concentrated bacterial culture;
- washing the concentrated bacterial culture with a sterile physiological saline;
- suspend the washed concentrated bacterial culture in a sterile physiological saline so as to obtain a bacterial suspension;
- adding to the bacterial suspension a sterilized kaolin suspension comprising kaolin dissolved in a sterile MSM culture solution. in a inoculation amount of 2˜8%, adsorbed and immobilized for 1˜4 hr, so as to prepare the kaolin immobilized GY2B bacteria.
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Dependencies are inferred from the claim text.