Improved nitrile hydratase
AssigneeMitsubishi Chemical Corporation
InventorsAya KITAHARA, Takanori AKIYAMA, Miki WAKAMATSU, Fumiaki WATANABE
Life of this patent
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Abstract
Provided is a novel improved nitrile hydratase with improved resistance to amide compounds under high temperatures. Specifically provided is a nitrile hydratase having at least one amino acid mutation selected from (a) to (d) below, in the amino acid sequence expressed in SEQ ID NO:50 (X1 to X27 represent independent arbitrarily-defined amino acid residuals). (a) X1 is valine or glycine (b) X9 is valine or threonine (c) X23 is an amino acid selected from a group consisting of isoleucine, leucine, methionine and threonine, (d) X24 is leucine.
Claims13
A nitrile hydratase having at least one amino acid sequence represented by the following SEQ ID NO:
- 46, 47, 48 or 49 in an α subunit:
- (a) SEQ ID NO:
- 46:
- X1X2X3X4X5X6RX7KAX8E
- wherein X1 is an amino acid other than tyrosine;
- X2, X3, X5, X6, X7 and X8 each independently are any amino acid residue;
- and
- X4 is tyrosine;
- (b) SEQ ID NO:
- 47:
- X9X10X11X12NX13X14X15X16X17X18X19X20X21CX22LC
- wherein X9 is an amino acid other than serine;
- X10, X11, X12, X13, X14, X15, Xis, X17, X19, X20, X21 and X22 each independently are any amino acid residue;
- and X18 is histidine;
- (c) SEQ ID NO:
- 48:
- X23WDSX25X26EX27RX28X29V
- wherein X23 indicates an amino acid other than valine;
- X25, X27, X28 and X29 each independently are any amino acid residue;
- and X26 is serine;
- (d) SEQ ID NO:
- 49:
- VX24DSX25X26EX27RX28X29V
- wherein X24 is an amino acid other than tryptophan;
- X25, X27, X28 and X29 each independently are any amino acid residue;
- and X26 is serine.
A nitrile hydratase having an amino acid sequence represented by SEQ ID NO:
- 50 in an α subunit, in which at least one amino acid mutation selected from the following (i) to (iv) is included:
- (i) X1 is glycine or valine,
- (ii) X9 is valine or threonine,
- (iii) X23 is an amino acid selected from the group consisting of isoleucine, leucine, methionine, and threonine, and
- (iv) X24 is leucine.
Dependencies are inferred from the claim text.