Mammalian chimeric complementation
AssigneeThe Salk Institute for Biological Studies
InventorsJuan Carlos IZPISUA BELMONTE, Jun WU
Life of this patent
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Abstract
Disclosed herein are chimeric mammals, such as a chimeric mammal comprising cells derived from at least a first mammal and a second mammal, wherein the cells from the first mammal comprise a genetic modification at one or more loci and the cells from the second mammal form at least one organ or tissue, wherein the first and second mammals are different species.
Claims26
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A mammalian organ or tissue isolated from a chimeric mammal comprising cells derived from at least a first mammal and a second mammal, wherein the organ or tissue is derived from an induced pluripotent stem cell (iPSC) cell from the second mammal, wherein the first and second mammal are different species.
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A method of making an induced pluripotent stem cell (iPSC) comprising:
- a) transfecting a population of human foreskin fibroblasts (HFF) with at least three episomal vectors comprising pCXLE-hOCT3/4-shp53-F, pCXLE-hSK, and pCXLE-hUL;
- b) contacting the transfected population of HFFs with a population of mitotically inactivated mouse embryonic fibroblasts (MEFs);
- c) contacting the population of HFFs and the population of MEFs with an FAC medium comprising a base media, an FGF, an Activin, and a WNT activator until at least one colony comprising an iPSC has formed;
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- d) transferring the colony comprising an iPSC to a fresh population of mitotically inactivated MEFs.
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Dependencies are inferred from the claim text.