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US 2017/0283774 A1Published application
Methods for efficient derivation of human motor neurons from diverse spinal regions
AssigneeWisconsin Alumni Research Foundation
InventorsEthan Scott Lippmann, Neha Sehgal, Randolph Scott Ashton
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Abstract
Described herein are chemically defined, adherent culture protocols for generating functional motor neurons characteristic of diverse hindbrain and spinal cord regions, with high efficiency.
Claims23
A method for generating motor neuron progenitor cells, comprising the steps of:
- i. transiently exposing SOX2− and Brachyury+ neuromesodermal progenitor cells cultured in a neural differentiation base medium that comprises FGF and a first concentration of a Wnt/β-catenin signaling pathway agonist to a second higher concentration of the agonist until NKX6.1+ ventral progenitor cells are obtained;
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- ii. culturing the NKX6.1+ ventral progenitor cells in the neural differentiation base medium comprising a retinoid and at least one sonic hedgehog (SHH) signaling pathway agonist in the absence of the Wnt agonist until OLIG2+, NKX6.1+, and PAX6+ motor neuron progenitor cells are obtained.
Dependencies are inferred from the claim text.