Downregulation of sine/alu retrotransposon transcription to induce or restore proliferative capacity and/or pluripotency to a stem cell
AssigneeBuck Institute for Research on Aging
InventorVictoria V. LUNYAK
Life of this patent
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Abstract
In certain embodiments methods are provided for inducing and/or restoring and/or maintaining a non-senescent phenotype, or aspects thereof (e.g., proliferative capacity and/or pluripotency) in a mammalian cell. The methods typically involve reducing the level or activity of SINE/Alu retrotransposon transcripts in the cell in an amount sufficient to induce or restore proliferative capacity and/or pluripotency to said mammalian cell.
Claims39
A method of inducing or restoring or maintaining proliferative capacity and/or pluripotency in a mammalian cell, said method comprising:
- reducing the level or activity of SINE/Alu retrotransposon transcripts in said cell in an amount sufficient to induce or restore proliferative capacity and/or pluripotency to said mammalian cell.
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A cell containing:
- a construct that reduces the level or activity of SINE/Alu transcripts in said cell whereby said cell has restored or maintains proliferative capacity and/or pluripotency;
- and/or
- a construct that reduces the level or activity of SINE/Alu transcripts and said cell shows reduced senescence as compared to a stem cell of the same type lacking said construct.
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A stem cell line, said cell line comprising stem cells containing a construct that stably down-regulates SINE/Alu transcripts.
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Dependencies are inferred from the claim text.