Processing of plant material into bacterial feedstock
AssigneeShanghai Zhiyi Information Technology Ltd.
InventorFeng Hong
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Abstract
Methods and compositions relating to processing of plant material into bacterial feedstock, such as bacterial feedstock suitable for nanocellulose production, are disclosed. Cellulosic plant fiber may be contacted with a catalyst such as an acid catalyst or an enzymatic catalyst or both, and the mixture can be hydrolyzed into a hydrolysate. The hydrolysate may be used to form a culture medium which can be used to support bacterial growth to form the nanocellulose.
Claims79
A method of making bacterial nanocellulose, the method comprising:
- contacting a cellulosic plant fiber with a catalyst selected from an acid catalyst, an enzymatic catalyst or a combination thereof to form a reaction mixture;
- subjecting the reaction mixture to hydrolyze at least a portion of the cellulosic plant fiber into a hydrolysate;
- forming a culture medium with the hydrolysate;
- inoculating the culture medium with at least one bacterium;
- and
- incubating the culture medium and the bacteria to produce the bacterial nanocellulose.
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A method of making a culture medium, the method comprising:
- contacting a cellulosic plant fiber with a catalyst selected from an acid catalyst, an enzymatic catalyst or a combination thereof, to form a reaction mixture;
- subjecting the reaction mixture to hydrolyze at least a portion of the cellulosic plant fiber into a hydrolysate;
- and
- forming the culture medium with the hydrolysate.
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A culture medium comprising:
- a hydrolysate prepared by contacting a cellulosic plant fiber, wherein the cellulosic plant fiber is Sorghum bagasse, sugar cane bagasse, or both, with a catalyst selected from an acid catalyst, an enzymatic catalyst or a combination thereof, to form a reaction mixture;
- and subjecting the reaction mixture to hydrolyze at least a portion of the cellulosic plant fiber into the hydrolysate, wherein the hydrolysate has a reducing sugar concentration of about 5 g/L to about 200 g/L.
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Dependencies are inferred from the claim text.