Cell culture monitoring system with low power consumption
OwnerHong Penginventor
Life of this patent
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Abstract
An improved cell culture monitoring system and method that detects cell growth and concentration in a dynamic environment of incubator/shaker. In order to reduce power consumption and make a wireless cell culture monitoring system practical, several methods of temperature compensation are used to replace a method of controlling the temperature of sensing module. Furthermore its power consumption can be significantly reduced by using an adaptive and synchronized light pulse detection technique.
Claims20
An apparatus for monitoring biological cell culture in a dynamic environment of a biological culture incubator/shaker, comprising:
- a container that can hold a liquid biological culture medium in which biological cells are incubated, and at least a part of the container's wall is optically transparent;
- at least one light emission source means for emitting light to interact with said biological culture medium through the transparent wall of said container;
- at least one photodetector means for directly detecting scattered or transmitted light by said biological culture medium through the transparent wall of said container when the emitted light from said light emission source interacts with said biological culture medium;
- at least one temperature sensor means for measuring temperature of said light emission source and said photodetector;
- temperature compensation means for reducing measurement error without temperature control of said light emission source and said photodetector;
- processing means for detecting and amplifying electrical signal from said photodetector, and then for processing the signal and presenting the measurable property values of the biological cell culture.
A method for monitoring biological cell culture in a dynamic environment of a biological culture incubator/shaker, comprising:
- utilizing a container to hold a liquid biological culture medium in which biological cells are incubated, and at least a part of the container's wall is optically transparent;
- positioning a light emission source relative to the transparent wall of said container and irradiating light through the wall of said container and interacting with said biological culture medium;
- positioning and aiming at least one photodetector to detect light from the interacting section of the incident light with the biological culture medium;
- positioning at least one temperature sensor close to said light emission source and said photodetector;
- providing temperature compensation to reduce measurement error without temperature control of said light emission source and said photodetector ;
- providing processing means for detecting and amplifying electrical signal from said photodetector, and for processing the signal and presenting the values of the measurable properties of the biological cell culture.
Dependencies are inferred from the claim text.