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US 2017/0283759 A1Published application
Nanocomposite Scaffold for the In Vitro Isolation of Cells
AssigneeRensselaer Polytechnic Institute
InventorsRyan James Gilbert, Elizabeth Hager Cothren, Gregory Patrick Desmond, Marc Andrew Burnette, Erica Victoria Hoey, Stephanie Christine Krom, Clarissa May Herman
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Abstract
The present invention is directed to devices, methods and kits for cell-specific sorting of cells from a mixed population, e.g., from a tumor sample. Aspects of the invention combine aligned, electrospun microfibers with drug-or protein-releasing nanospheres to isolate cancer cells from tumor biopsies.
Claims20
A cell culture apparatus for cell-type specific separation of a mixed population of cells, comprising:
- a substrate comprising a surface of aligned fibers;
- one or more areas of the substrate comprising a protein layer in contact with the aligned fibers;
- and
- one or more chemoattractants disposed on at least one of the one or more areas of the substrate.
1 more element
A method of sorting cells, comprising:
- providing a substrate comprising a surface of aligned fibers, one or more areas of the substrate comprising a protein layer in contact with the aligned fibers, and one or more chemoattractants disposed on at least one of the one or more areas of the substrate;
- and
- contacting the substrate with a mixed population of cells, whereby the one or more chemoattractants attract a specific population of cells from the mixed population such that the specific population migrates toward the chemoattractants.
Dependencies are inferred from the claim text.