Multi-chambers bioreactor, methods and uses

A polymeric multi-chamber bioreactor comprising

• at least a first and a second fluid-tight chamber for receiving respectively a first fluid culture medium and a second fluid culture medium comprising respectively a first and a second cell culture, and an opening between the first and second chamber for interchanges between the chambers,
• each chamber comprising at least an inlet and an outlet of fluid,
• the first and second chamber comprising respectively a first and a second cell culture scaffold,
• wherein the first and second scaffold are in contact,
• wherein the chamber and the scaffolds are arranged such that when the bioreactor is in use, an interchange of cells of the first, or of the second, or of both the first and the second cell culture occurs between the chambers through one or both scaffolds;
• wherein the pore size of the scaffolds is such to allow the interchange between the chambers of cells of the first cell culture, or of the second cell culture or of both the first and second cell culture.