Production of proteins in glutamine-free cell culture media
AssigneeGenentech, Inc.
InventorsMartin GAWLITZEK, Shun LUO, Christina Teresa BEVILACQUA
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Abstract
The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.
Claims40
A process for producing a polypeptide in a mammalian host cell expressing said polypeptide, comprising culturing the mammalian host cell in a production phase of the culture in a glutamine-free production culture medium containing asparagine.
The process of claim 86 wherein the mammalian host cell is a dhfr− CHO cell.
A ready-to-use glutamine-free cell culture medium for the production of a polypeptide in a production phase.
Dependencies are inferred from the claim text.