US 2017/0283486 A1 · STABLE IMMUNOGEN BASED ON INNER DOMAIN OF HIV-1 GP120 FOR INDUCING IMMUNITY AGAINST HIV

Abstract

Stable immunogens comprising portions of the inner domain (ID) of the Human Immunodeficiency Virus (HIV-1) gp120 protein are provided. These ID immunogens selectively present the gp120 C1/C2 region in its CD4-bound state within a minimal structure. These ID immunogens can be used to vaccinate subjects against infection with HIV, the causative agent of Acquired Immunodeficiency Syndrome (AIDS). The immunogens can also be used as targets in screening to identify small molecule or peptide inhibitors that block HIV-1 viral entry and attachment steps, and as probes for identifying gp120 C1/C2 region-specific antibodies.

Claims21

Dependent claims

A HIV-1 gp120 inner domain (ID) immunogen, where the ID immunogen comprises:

(a) a mature human immunodeficiency virus 1 (HIV-1) gp120 polypeptide having two or more of the features (i)-(iv):
(i) deletion of amino acids 1-11 of the N-terminus,
(ii) cysteine residues at amino acids positions 65 and 115,
(iii) deletion of amino acids 258-472 of the outer domain region and replacement by a GlyGly dipeptide linker, and
(iv) deletion of amino acids 119-205 of the V1V2 loop and replacement by a GlyGlyAla tripeptide linker,
wherein the amino acid numbering corresponds to the amino acid sequence set forth in SEQ ID NO:1;
or
(b) a variant having at least about 95% sequence identity with an immunogen of (a) over the entire length of the immunogen sequence.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/504,770Filed 19 Aug 2015

Priority

20 Aug 2014

Published

5 Oct 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification21 codes

C07K 16/1145
Lentivirus , e.g. human immunodeficiency virus [HIV], feline immunodeficiency virus [FIV] or simian immunodeficiency virus [SIV]: env proteins,…
A61K 39/12
Medicinal preparations containing antigens or antibodies: viral antigens
A61K 39/21
Viral antigens: retroviridae, e.g. equine infectious anemia virus
A61K 39/385
Medicinal preparations containing antigens or antibodies: haptens or antigens, bound to carriers

Stable immunogen based on inner domain of hiv-1 gp120 for inducing immunity against hiv

Life of this patent

Stable immunogen based on inner domain of hiv-1 gp120 for inducing immunity against hiv

Life of this patent

Abstract

Claims21