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US 2017/0283451 A1Published application
Dna sequencing by synthesis using rama and ifrared spectroscopy detection
AssigneeThe Trustees of Columbia University in the City of New York
InventorsJingyue JU, Jian WU, Zengmin LI
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Abstract
This invention provides a process of labeling a polynucleotide analogue to be detected by Raman and/or infrared spectroscopy detection.
Claims14
A nucleoside triphosphate analogue having the structure:
- wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is OH or H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl other than aziodmethyl, which has a Raman spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1 or a Fourier transform-infrared spectroscopy peak with wavenumber from 2000 cm−1 to 2300 cm−1.
2-5. (canceled)
A polynucleotide analogue, wherein the polynucleotide analogue differs from a polynucleotide by comprising at its 3′ terminus one of the following structures in place of the H atom of the 3′ OH group normally present in the polynucleotide:
- wherein the wavy line indicates the point of attachment to the 3′ oxygen atom.
9-19. (canceled)
A nucleoside triphosphate analogue having the structure:
- wherein the base is adenine, guanine, cytosine, uracil or thymine,
- wherein R″ is an OH or an H,
- wherein L a cleavable linker, and
- wherein R has the structure:
- wherein the wavy line indicates the point of attachment to L.
2 more elements
22-25. (canceled)
27-28. (canceled)
31-76. (canceled)
Dependencies are inferred from the claim text.