Method for extracting high-purity asarinin by supercritical carbon dioxide extraction method and use thereof

A method for extracting high-purity asarinin by an SFE-CO2 method, characterized in that:

• the method comprises the operating steps below:
• (1) washing roots and/or rootstocks of asarum, airing and smashing to obtain crude asarum powder;
• (2) putting the crude asarum powder in an extraction kettle of an SFE-CO2 device and sealing;
• respectively heating the extraction kettle and separating kettles, opening a valve of the extraction kettle when the extraction kettle reaches a temperature of 45-70° C., and the separating kettles I and II reach a temperature of 30-50° C., opening an exhaust valve to empty air when a pressure in the extraction kettle is equal to that of a storage tank, pressurizing a system by a high-pressure pump, and controlling a flow rate of CO2 to 35-60 L/h to start cycling extraction when the extraction kettle reaches a pressure of 30-40 Mpa, and the separating kettles I and II reach a pressure of 5-10 Mpa;
• and extracting for 60-240 min to obtain total volatile oil of asarum;
• (3) standing the total volatile oil of asarum for 24-72 h at a low temperature of −20-4° C., and carrying out suction filtration at reduced pressure to obtain crude asarinin;
• and
• (4) dissolving the crude asarinin with absolute ethyl alcohol with 1-5 times of volume dosage of the crude asarinin, and recrystallizing at a low temperature of −20-4° C. to obtain pure asarinin with a quality purity greater than 98% until white acicular crystals are precipitated.